Agrisera/MTP1 | vacuolar Zn2+/H+ antiporter/AS09 485/-免疫在线蚂蚁淘旗下平台-

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Agrisera/MTP1 | vacuolar Zn2+/H+ antiporter/AS09 485/

来自 : 发布时间：2024-12-23


product information
Background		MTP1 is mediating zinc accumulation in roots and resistance to zinc. Is involved in sequestering of zinc from cytoplasm into vacuole in conditions of its excess. Alternative names: Zinc transporter ZAT-1, ZAT1p, 43.8 \| metal tolerance protein 1
Immunogen		KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MTP1 Q9ZT63, At2g46800
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format
Quantity		100 µl
Reconstitution
Storage		store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications		ELISA (ELISA), western blot (WB)
Related products		collection of antibodies to plant tonoplast membrane proteins AS09 474 \| anti-AtMTP4 \| metal tolerance protein B Plant protein extraction buffer Secondary antibodies
Additional information		0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable. MTP1 protein is of low abundance in plant tissues. Protocol for isolation of tonoplast proteins can be found here.

application information
Recommended dilution		1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
Expected \| apparent MW		43.8 \| 43 kDa (Arabidopsis thaliana)
Confirmed reactivity		Arabidopsis thaliana, Nicotiana tabacum
Predicted reactivity		dicots including: Brassica sp. , Noccaea fendleri, Thlaspi caerulescens,
Not reactive in		members of Solanaceae
Additional information		Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Selected references		Vera-Estrella et al. (2017). Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017 Apr 28. doi: 10.1007/s00425-017-2700-1. Kawachi et al. (2008). Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, stimulates the transport activity. J.Biol. Chem. 13:8374-8383. Kobae et al. (2004). Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol. 12:1749-1758.

application example

sample of a vector (1) and vacuolar membrane fraction of yeast cells expressing AtMTP1 (2) separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AtMTP1 antibodies (AS09 485, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

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发布于： 2024-12-23 阅读（）

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