Agrisera/V-ATPase, B | vacuolar H+-ATPase subunit B/AS14 2775/-免疫在线蚂蚁淘旗下平台-

当前位置：首页 > 新闻动态 >

热卖商品

Agrisera/PetC | Rieske iron-sulfur protein of Cyt b6/f complex, protein standard for quantitation of PetC protein/AS08 330S

Agrisera/Exon4b/AS15 2986/

Agrisera/Anti-RGA-like protein 2 | DELLA protein RGL2 ANTIBODY/AS11 1803

Agrisera/抗GSNOR | S-亚硝基谷胱甘肽还原酶抗体/AS09 647

新闻详情

Agrisera/V-ATPase, B | vacuolar H+-ATPase subunit B/AS14 2775/

来自 : 发布时间：2024-05-13


product information
Background		V-ATPase, subunit B is a non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. Alternative names: Vacuolar proton pump subunit B1, vacuolar H+-ATPase subunit B, V-ATPase 57 kDa subunit.
Immunogen		KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana V-ATPase subunit B, isoform B1: UniProt: Q683E8, TAIR: AT1G76030, isoform B2 UniProt: Q9SZN1, TAIR: AT4G38510, isoform B3: UniProt: Q8W4E2 , TAIR: AT1G20260
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Affinity purified serum in PBS pH 7.4
Format
Quantity		50 µg
Reconstitution
Storage		store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications		western blot (WB)
Related products		collection of antibodies to vacuolar proteins AS09 467 \| anti-V-ATPase subunit A \| vacuolar H+-ATPase AS09 468 \| anti-V-ATPase subunit c \| vacuolar H+-ATPase, subunit c (16 kDa) AS09 497 \| anti- V-ATPase subunit D \|V-type proton ATPase subunit D AS07 213 \| anti-V-ATPase subunit E of tonoplast H+ATPase AS09 499 \| anti-V-ATPase subunit H \|V-type proton ATPase subunit H Plant protein extraction buffer Secondary antibodies
Additional information		Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient. Protocol for isolation of vacuolar membranes can be found here.

application information
Recommended dilution		1: 1000 with ECL (WB)
Expected \| apparent MW		53 \| 57 kDa (Vigna radiata)
Confirmed reactivity		Arabidopsis halleri, Arabidopsis thaliana, Nicotiana tabaccum , Thellungiella salsuginea, Vigna radiata
Predicted reactivity		Acetabularia sp. , Arundo donax, Chlamydomonas reinhardtii, Glycine soja, Gossypium mexicanum, Halostachys caspica, Haloxylon ammodendron, Hordeum vulgare, Medicago truncatula, Mesembryantheum crystallinum, Ostreococcus tauri, Oryza sativa, Panax ginseng, Physcomitrella patens, Pinus sylvestris, Populus trichocarpa, Pyrus sp., Ricinus communis, Theobroma cacao, Triticum aestivum, Zea mays, Zostera marina
Not reactive in		no confirmed exceptions from predicted reactivity known in the moment
Additional information		Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Selected references		to be added when available, antibody released in June 2015

applicaiton example $\"western$ Proteins were separated by SDS-PAGE and transferred to an Immobilon-P membrane (Millipore) using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) with transfer buffer (100 mM Tris, 192 mM Glycine, 0.02% (w/v) SDS and 5% (v/v) methanol). After treatment with 1% ECL blocking agent (GE Healthcare), the membrane filter was incubated with the primary antibody (1:1000) and then with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (H+L) (Agrisera AS09 602, 1:25 000). Chemiluminescent reagent ECL (GE Healthcare) was used for detection of antigens. Chemiluminescence was detected with a Light-Capture II imaging device with a cooled CCD camera (Atto). Samples: 1: 10 μg of 100,000 x g precipitate prepared from Arabidopsis thaliana 6 weeks old shoot. 2: 0.2 μg of vacuolar membrane enriched fraction prepared from Arabidopsis thaliana 6 weeks old shoot. 3: 2 μg of vacuolar membrane enriched fraction prepared from Arabidopsis thaliana 6 weeks old shoot. 4: 0.2 μg of vacuolar membrane enriched fraction prepared from Vigna radiata 4 days old hypocotyls. 5: 2 μg of vacuolar membrane enriched fraction prepared from Vigna radiata 4 days old hypocotyls. Courtesy of Drs. Masayoshi Maeshima and Dr Shoji Segami , Nagoya University, Japan

本文链接： http://agrisera.immuno-online.com/view-1454212097.html

发布于： 2024-05-13 阅读（）

没有了