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Agrisera/Goat anti-Rat IgG (H&L), DyLight® 550 conjugated/AS12 1977/
来自 : 发布时间:2024-12-22
product information
Background

Goat anti-Rat IgG (H&L) - Affinity Pure, DyLight®550 Conjugateis a secondary antibody conjugated to DyLight® 550, which binds to allratIgG(H&L)in immunological assays.

DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phaserat IgG (H&L).

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Immunogen

Purified rat IgG

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified Goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products

other dylight anti-rat secondary antibodies

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

application information
Recommended dilution

1:20- 1:2,000 for most applications

Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information

Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.

No reactivity is observed to: non-immunoglobulin rat serum proteins.

Selected references

to be added when available


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发布于 : 2024-12-22 阅读()