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Agrisera/PetC | Rieske iron-sulfur protein of Cyt b6/f complex, protein standard for quantitation of PetC protein/AS08 330S
Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2μg of chlorophyll will give a PsbA signal in this range.
Positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
33 kDa (larger than native protein due to the addition of His-tag). In most gel systems, PetC protein migrates at 23 kDa
