Agrisera/AtpF | CF0I subunit of ATP synthase/AS10 1604/-免疫在线蚂蚁淘旗下平台-

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Agrisera/AtpF | CF0I subunit of ATP synthase/AS10 1604/

来自 : 发布时间：2024-05-13


product information
Background		The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Immunogen		isolated CF₀I subunit of the chloroplast ATP synthase complex
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Liquid
Quantity		100 µl
Reconstitution
Storage		Short term 4°C. Long term -20°. Repeated freezing and thawing is not recommended. Solution contains 0.01% sodium azide as preservative.
Tested applications		western blot (WB)
Related products		AS08 304 \| AtpA \| alpha subunit of ATP synthase antibody AS03 030 \| ATP synthase subunit beta hen antibody AS05 085 \| ATP synthase subunit beta rabbit antibody AS10 1590 \| AtpD \| CF1 delta subunit of ATP synthase antibody (algae) AS10 1591 \| AtpD \| CF1 delta subunit of ATP synthase antibody (highr plants) AS10 1583 \| AtpI \| CFoIV subunit of ATP synthase antibody AS08 312 \| anti-ATP synthase subunit gamma antibody AS09 591 \| AtpH \| ATP synthase subunit c antibody AS05 071 \| AtpH \| ATP synthase subunit c antibody AS08 370 \| ATP synthase whole enzyme antibody Secondary antibodies
Additional information		This product can be sold containing proClin if requested.

application information
Recommended dilution		1: 5000 with standard ECL (WB)
Expected \| apparent MW		21 kDa
Confirmed reactivity		Arabidopsis thaliana, Spinacia oleracea, Chlamydomonas reinhardtii
Predicted reactivity		higher plants
Not reactive in		no confirmed exceptions from predicted reactivity known in the moment
Additional information		to be added when available
Selected references		Grieco et al. (2015). Light-harvesting II antenna trimers connect energetically the entire photosynthetic machinery - including both photosystems II and I. Biochim Biophys Acta. 2015 Jun-Jul;1847(6-7):607-19. doi: 10.1016/j.bbabio.2015.03.004. Epub 2015 Apr 3. Yap at al. (2015). AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5 and also promotes atpF splicing in Arabidopsis and rice. Plant J. 2015 Jan 13. doi: 10.1111/tpj.12756.

application example $\"western$ 20 µg of chloroplast fraction from Arabidopsis thaliana (1) and Spinacia oleracea (2) were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 2 % non-fat milk powder in 1xTBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions.

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发布于： 2024-05-13 阅读（）

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