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Agrisera/AgriseraTMB HRP Peroxidase Microwell Substrate/AS15 Diluent
Agrisera/AgriseraTMB HRP Peroxidase Microwell Substrate/AS15 Diluent
商品编号：
AS15Diluent
品牌：
Agrisera AB
市场价：
¥1882.40
美元价：
1129.44
产品分类：
多克隆抗体
公司分类：
Polyclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Quantity100 ml
StorageStable for 3 years at +2-8° C. It can also be stored at room temperature up to six months, and can also be frozen. Protect the substrate from exposure to sunlight.
Tested applicationsInstructions for a 96 well micro titer plate 1. Equilibrate the substrate to the assay temperature before use. 2. Complete all required incubations with antibodies and HRP labeled reagents. 3. Wash the plate at least 4 times with phosphate buffered saline or Tirs buffered saline containing 0.1% Tween-20. THen shake and blot all residual buffer from plate wells. 4. Add 100 µl of the TMB solution to each well and incubate 5-30 minutes, depending upon the activity of the HRP probe. If color develops too rapidly, zero order kinetics will not prevail. Dilution of antibody, or HRP-labeled reagent may be required. As alternative Agrisera TMB/Diluent pack can be used. 5. To stop the reaction use:-equial volume of 0.3 M sulfuric acid (100 µl). The yellow chromogen in the stopped reaction should be read at 450 nm. This will provide highest sensitivity of detection. -Monitoring the assay kinetics at may be done at 650 nm as a function of time. The reaction can be stopped to preserve the blue chromogen using an equal volume, 100 µl of 0.1% sodium fluoride or 0.15% sodium dodecyl sulfate. The blue chromogen in the stopped reaction should be measured at 650 nm. Adjustments of time, reagent volume and temperature require standardization by the end user. In case of any further questions please contact us.
品牌介绍
Agrisera针对植物蛋白质的保守氨基酸序列，Agirsera还**开发了数十种通用抗体（global antibodies），这类抗体几乎可以与所有的高等植物及藻类产生免疫反应，可为研究人员提供极为方便的选择。其中包括常用的植物Rubisco抗体，Rubisco即核酮糖二磷酸羧化酶，是植物组织中广泛存在的一种高丰度蛋白，在一些植物学WB检测时，经常被用作内参抗体使用。
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