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当前位置: 首页 > 产品中心 > Other_natural_proteins > Agrisera/RE|N端精氨酸化/AS15 3050/
商品详细Agrisera/RE|N端精氨酸化/AS15 3050/
Agrisera/RE|N端精氨酸化/AS15 3050/
Agrisera/RE|N端精氨酸化/AS15 3050/
商品编号: AS153050
品牌: Agrisera AB
市场价: ¥10705.80
美元价: 6423.48
产地: 美国(厂家直采)
公司:
产品分类: 其它天然蛋白
公司分类: Other_natural_proteins
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
productinformation
BackgroundArginylationisapost-translationalmodificationof anexistingpeptidechainbyadditionofanextraarginine.Thismodificationchangesprimarysequenceofproteinaswellasit"ssurfacechange.ItismediatedbyarginyltransferaseATE1.Arginylationplaysanessentialroleinmultiplephysiologicalpathways,forexample,invivoarginylationconstitutesamechanismfordegradationofpreprocessedproteinsorproteolyticfragmentsthatbearAspandGluontheirN-termini.
Immunogen

KLH-conjugatedsyntheticpeptide:H-REHKHANQHMSVC-NH2

HostRabbit
ClonalityPolyclonal
Clone
PurityAffinitypurifiedseruminPBSpH7.4
Format
Quantity2x50µg
Reconstitution

Forreconstitutionadd50µlofsterilewatertoeachtube

Storage

Storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.

TestedapplicationsDotblot(Dot)
Relatedproducts

AS153050A|anti-RD|N-terminalarginylation,rabbitantibodies

collectionofantibodiestoproteinsinvolvedintranscription
collectionofantibodiestoproteinsinvolvedintranslation

Plantproteinextractionbuffer

Secondaryantibodies

AdditionalinformationAntibodyispurifiedusingsubstractivepurificationmethod. 
applicationinformation
Recommendeddilution

1: 1000(Dot)

Expected|apparentMW
Confirmedreactivity
Predictedreactivity
Notreactivein
AdditionalinformationMG132orepoxomycinarerecommendedtousetoinhibitproteasomeandsignificantlyincreasesignalfromthearginylatedproteins.
Selectedreferences

Tobeaddedwhenavailable,antibodyreleasedinJune2016.


Applicationinformation

Dot blot using anti-arginylation antibodies

  • Membrane:PVDF,Hybond0.45μm(GEHealthcare)
  • 0.5µgsyntheticpeptideperspot
  • MembranewasblockedovernightinTBST,4%BlockingAgent(GEHealthcare)
  • Primaryantibodies:1:10000inTBST2%BlockingAgent
  • Secondaryantibody(AS09602):1:25000inTBST2%BlockingAgent
  • Detection:ECLAdvance(GEHealthcare)


CourtesyofDr.SebastianHoernstein,FacultyofBIOLOGy,UniversityofFreiburg;Germany

品牌介绍
Agrisera针对植物蛋白质的保守氨基酸序列,Agirsera还**开发了数十种通用抗体(global antibodies),这类抗体几乎可以与所有的高等植物及藻类产生免疫反应,可为研究人员提供极为方便的选择。其中包括常用的植物Rubisco抗体,Rubisco即核酮糖二磷酸羧化酶,是植物组织中广泛存在的一种高丰度蛋白,在一些植物学WB检测时,经常被用作内参抗体使用。