| productinformation
| | Background | | Xyloglucansarepolysaccharidescommonlyreferedtoashemicellulosesfoundintheprimarycellwallsofvascularplants.Thisantibodybindstogalactosylatedside-chainsofnon-fucosylatedxyloglucan-5,andappearstopreferentiallybindtothegalactosylatedside-chainclosesttothereducingendofxyloglucanoligosaccharidesub-units(XXLG,XLLG). | | Immunogen | | BSA-conjugatedtamarindxyloglucan(covalent).Epitopestructureforcarbohydrateantigen:XXLG,XLLG. | | Host | | Mouse | | Clonality | | Monoclonal | | Clone | | IgG1,cloneCCRC-M48 | | Purity | | | | Format | | Cellculturesupernatant,liquid. | | Quantity | | 5ml | | Reconstitution | | | | Storage | | Antibodycanbestoredupto1monthat4°C,andat-80°Cforupto 1year.
Makealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromliquidmaterialadheringtothecaporsidesofthetubes. | | Testedapplications | | ELISA(ELISA),immunohistochemistry(IHC),immunofluorescence(IF) | | Relatedproducts | | AS163115|anti-ArABInogalactan-2(CCRC-M133),mousemonoclonalantibody
AS163133|anti-Arabinogalactan-3(CCRC-M85),mousemonoclonalantibody
AS163231|anti-Arabinogalactan-4(CCRC-M78),mousemonoclonalantibody
AS163226|anti-Acetylatedglucomannan(CCRC-M170),mousemonoclonalantibody
AS163116|anti-Galactomannan-1(CCRC-M70),mousemonoclonalantibody
AS163232|anti-Galactomannan-2(CCRC-M174),mousemonoclonalantibody
AS163117|anti-Homogalacturonan-1(CCRC-M38),mousemonoclonalantibody
AS163136|anti-Fucosylatedxyloglucan(CCRC-M1),mousemonoclonalantibody
AS163230|anti-Non-fucosylatedxyloglucan-1(CCRC-M101),mousemonoclonalantibody
AS163233|anti-Non-fucosylatedxyloglucan-2(CCRC-M88),mousemonoclonalantibody
AS163234|anti-Non-fucosylatedxyloglucan-3(CCRC-M100),mousemonoclonalantibody
AS163235|anti-Non-fucosylatedxyloglucan-4(CCRC-M58),mousemonoclonalantibody
AS163225|anti-Rhamnogalacturonan-I/Arabinogalactan(CCRC-M7),mousemonoclonalantibody
AS163224|anti-Rhamnogalacturonan-Ibackbone(CCRC-M35),mousemonoclonalantibody
AS163134|anti-Rhamnogalacturonan-Ia(CCRC-M2),mousemonoclonalantibody
AS163237|anti-Rhamnogalacturonan-Ib(CCRC-M61),mousemonoclonalantibody
AS163236|anti-Rhamnogalacturonan-Ic(CCRC-M23),mousemonoclonalantibody
AS163135|anti-Xylan-1/Xyloglucan(CCRC-M108),mousemonoclonalantibody
AS163227|anti-Xylan-3(CCRC-M114),mousemonoclonalantibody
AS163238|anti-Xylan-4(CCRC-M154),mousemonoclonalantibody
AS163239|anti-Xylan-5(CCRC-M144),mousemonoclonalantibody
AS163229|anti-Xylan-6(CCRC-M138),mousemonoclonalantibody
AS163228|anti-Xylan-7(CCRC-M149),mousemonoclonalantibody Plantandalgalproteinextractionbuffer Secondaryantibodies | | Additionalinformation | | exactworkingdilutionneedstobedeterminedbyenduser |
applicationinformation
| | Recommendeddilution | | undilutedorat1:10 | | Expected|apparentMW | | | | Confirmedreactivity | | Arabidopsisthaliana,Camelinasativa,Tamarindusindicus | | Predictedreactivity | | dicots | | Notreactivein | | noconfirmedexceptionsfrompredictedreactivityarecurrentlyknown | | Additionalinformation | | CCRC-M48bindstogalactosylatedside-chainsofnon-fucosylatedxyloglucan,andappearstopreferentiallybindtothegalactosylatedside-chainclosesttothereducingendofxyloglucanoligosaccharidesub-units(XXLG,XLLG). | | Selectedreferences | | tobeaddedwhenavailable,antibodyreleasedinMarch2016. |
Applicationexample
Localizationofnon-fucosylatedxyloglucan-5(red)inArabidopsisthalianahypocotyl,CalcufluorWhitecounterstain(blue)andcellwallautofluorescence(yellow).
The31days-oldhypocotylswereimmersedin150μLPMEfixationbuffer(25mMPIPES,1mMMgSO4,1mMEGTA)andthensubjectedtothreeconsecutivecyclesof5min-longvacuuminfiltration(21°C,68kPa).AfterwardstheywerewashedthreetimesinPME(21°C,68kPa)priortostorageat4°CinPME.Hypocotylswereencasedin1cm3blocksof5%agarat65°C,andstoredat4°Ctoset.Transverse40μmthicksectionswerecutfromsegmentsusingaVT100Svibratingmicrotome(Leica)andblockedforatleast1hin5%bovineserumalbumininTBST.Blockingsolutionwasdiscardedandsectionswereincubatedat4°Cfor16hwith5μloftheanti-Non-fucosylatedxyloglucan-5antibody,followedby2washesin100μLTBST.Sectionswerethenincubatedfor1hat21°Cinthedarkin10μlof2μg/μlAlexaFluorTM568donkeyanti-mouseIgG(H+L;1:36).Sectionswereagainwashedtwicein40μLTBSTpriortocounter-stainingwith0.015%CalcofluorWhite(Sigma-Aldrich).Sectionswereagainwashedtwicein100μLTBSTtoremoveexcesscounter-stainandunboundsecondaryantibody.ImmunofluorescenceofAlexaFluor568wasexcitedwitha561nmlaser,andemittedlightfilteredat575–600nm,whileCalcufluorWhitewassubsequentlyscannedonanindependentchannelwitha405nmlaserandemissionobservedat420–430nmusinglaserscanningmicroscopeZeissLSM780point-scansystemat1024×1024pixels(pixelsize,0.6–0.83μm)witha10Xobjective.
CourtesyDr.UrsFisher,UmeåPlantScienceCentre,Sweden
品牌介绍
Agrisera针对植物蛋白质的保守氨基酸序列，Agirsera还**开发了数十种通用抗体（global antibodies），这类抗体几乎可以与所有的高等植物及藻类产生免疫反应，可为研究人员提供极为方便的选择。其中包括常用的植物Rubisco抗体，Rubisco即核酮糖二磷酸羧化酶，是植物组织中广泛存在的一种高丰度蛋白，在一些植物学WB检测时，经常被用作内参抗体使用。
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