PSII蛋白质标准/AS09 146S的Agrisera/PsbD | D2蛋白质
商品编号：
AS09146S
品牌：
Agrisera AB
市场价：
¥6211.80
美元价：
3727.08
产品分类：
其它天然蛋白
公司分类：
Other_natural_proteins
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
FormatLyophilized in glycerol
Quantity250 µl
ReconstitutionFor reconstitution add 225 µl of sterile water. Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
StorageStore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applicationsWestern blot (WB)
Recommended dilution
Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2 μg of chlorophyll will give a PsbD signal in this range.
Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems.
This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Expected | apparent MW
In most gel systems PsbD migrates around 28-30 kDa
品牌介绍
Agrisera针对植物蛋白质的保守氨基酸序列，Agirsera还**开发了数十种通用抗体（global antibodies），这类抗体几乎可以与所有的高等植物及藻类产生免疫反应，可为研究人员提供极为方便的选择。其中包括常用的植物Rubisco抗体，Rubisco即核酮糖二磷酸羧化酶，是植物组织中广泛存在的一种高丰度蛋白，在一些植物学WB检测时，经常被用作内参抗体使用。
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